Soluble Mediators From Lactobacillus rhamnosus GG Reduce Intestinal Permeability And Bacterial Translocation In a Rat Model of Short Bowel Syndrome Model

Objectives and studyThe aim of this study was to evaluate the potential effects of specific preparations of soluble mediators derived from Lactobacillus rhamnosus GG (LGG) on intestinal barrier function in a rat model of short bowel syndrome (SBS). Methods6-week old male SD rats were randomized into experimental groups (n=8). Sham operated rats underwent bowel transection and re-anastomosis. The remaining animals underwent 75% small bowel resection and received either supplementation of 5*108 CFU viable LGG, two different LGG soluble mediator preparations in an equivalent dose only differing in the desalting methodology applied (ultrafiltration vs. column chromatography), or PBS by intragastric gavage daily from day 2 throughout day 14 after small bowel resection. Body weight of the animals was measured regularly. On day 15, intestinal permeability (fluorescein isothiocyanate-dextran-40,000 Daltons) and bacterial translocation to mesenteric lymph nodes, liver and spleen were measured. Serum levels of endo...