Phylogenetic analysis of Engleromyces sinensis and identification of cytochalasin D from culture

Engleromyces sinensis (Xylariales, Xylariaceae) is an important medicinal fungus in China. In the present study, its taxonomic status was confirmed by muti-locus DNA (ITS-LSU-RPB2-TUB2) phylogenetic analysis for the first time. The main active components of the stromata and cultured mycelium of E. sinensis were analyzed by high-performance liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) methods. Cytochalasin D was identified in stromata and mycelium, and no neoengleromycin was detected. Due to the lack of molecular data related to E. goetzei, the interspecific relationships of E. goetzei and E. sinensis need to be investigated further.