꿀벌 병원성 바이러스의 정량적 검출을 위한 정량 실시간 재조합효소-중합효소 증폭법의 개발

Recombinase polymerase amplification (RPA) is a specific DNA amplification method which shows high speed in isothermal condition and it has been widely applied to the detection of various pathogens. In this study, kSBV specific real-time recombinase polymerase amplification method was newly developed for rapid detection of kSBV. The existence of kSBV specific gene could be detected using total cDNA within 2 min 46 sec using this method. Based on this method, we proposed the quantitative real-time recombinase polymerase amplification which was able to quantify the target gene and to be applied universally. Moreover, this assay was proved to be useful to detect the pathogen from real samples. It is expected that this assay will be applied as quantitative detection method for general pathogens as well as honeybee pathogens.