Morphological and immunological diagnosis of chronic myeloid leukemia in different phases

Objective: To study the combination of morphological and immunophenotype diagnosis in chronic myeloid leukemia at different phases. Methods: Indirect immunofluorescence assay was used to detect the immunophenotype of bone marrow mononuclear cells in 80 CML patients. Results: The expression rates of CD 34 and HLA DR were 3.6% and 31% in the chronic phase, 36% and 73% in acceleration phase, 62% and 100% in blastic phase, respectively. Both CD 34 and HLA DR were significantly higher in blastic phase than chronic phase (P0.05). CD 15 \++ cells tend to decrease with the progression of the disease and were especially significantly lower in blastic phase than that in chronic and acceleratad phase (P0.05). CD\-9\++ cells were detected in 11/13 patients in blastic phase. The diagnostic coordination rate between immunophenotype and morphology was 77% in 13 CML patients in blastic phase. Blast cells expressed both lymphoid and myeloid lineage antigens in part of blastic patients. Conclusion: The membrane markers have determinant significance to identify the type of blast crisis and progression of the disease in different phases,but morphology and cytochemistry has its important role which can't be replaced by immunophenotyping. The combination of morphology、 cytochemistry and immunophenotyping can complement each other in the diagnosis of CML.