Methods for studying the function of cytomegalovirus GPCRs.

: All of the cytomegaloviruses discovered to date encode two or more genes with significant homology to G-protein coupled receptors (GPCRs). The functions of these cytomegalovirus GPCRs are just beginning to be elucidated; however, it is clear that they exhibit numerous interesting activities in both in vitro and in vivo systems. In this chapter, we review the various methodologies that can be used to examine biochemical aspects of viral GPCR signaling in vitro as well as examine the biological activity of these viral GPCRs in vitro and in vivo in virus infected cells using recombinant cytomegaloviruses.