Erythropoietin Receptor in Human Tumor Cells: Expression and Aspects Regarding Functionality
2001
textabstractRecombinant human erythropoietin (Epo)and granu l o cy t e - c o l o ny - s t i mulating factor (G-CSF) are used
to stimulate hematopoiesis in patients with malignant dise
a s e s . These cytokines transduce their biological signal via
the Epo receptor (EpoR) and G-CSF receptor (G-CSF-R) into
the cell. We therefore investigated in human tumor cell lines
the expression of these receptors in tumor cells as well as
their response to Epo and G-CSF.
Methods and study design: The expression of EpoR and G-CSF-R
mRNA was analy zed with rev e rse transcription-poly m e r a s e
chain reaction (RT-PCR). EpoR protein expression was further
monitored with Western blot and immunocytochemistry analys
i s . The cellular response to various concentrations of Epo
was evaluated using 3[H]-thymidine uptake, Northern blot of cfos
expression and tyrosine kinase activity assay. The proliferation
after G-CSF incubation was analyzed with the MTS assay.
Results: In this study EpoR mRNA and protein were detected in
various human tumor cell lines. Treatment with Epo did not influence
the pro l i feration rate of examined EpoR-positive tumor
cell lines.Epo did not stimulate the tyrosine kinase activity
nor did it affect the c-fos mRNA in these cell lines.G-CSF-R
mRNA was only detected in two myeloid cell lines. Treatment
with G-CSF did not increase the proliferation of these cells.
C o n c l u s i o n s : These results demonstrate that Epo and G-CSF did
not modulate the growth rate of examined receptor-positive tumor
cell lines; the presence of the Epo receptor seems not essential
for cell growth of these tumor cells in cell culture.
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