Study on the interaction between methyljasmonate and CuZn-superoxide dismutase by spectroscopic methods

The interaction between CuZn-superoxide dismutase(SOD) and methyljasmonate(MeJA) was studied by fluorescence and UV-Vis spectroscopic techniques.The quenching mechanism of fluorescence of MeJA by SOD was discussed to be a static quenching procedure.The number of binding sites n and apparent binding constant K was measured by fluorescence quenching method.The thermodynamics parameter ΔH,ΔG,ΔS were also calculated.The results indicate the binding reaction was both entropy-driven and the enthalpy-driven,and the electrostatic and hydrophobic binding played major role in the interaction.The binding sites of MeJA with SOD were found to approach the microenvironment of both Trp and Tyr by the synchronous fluorescence spectrometry.The distance r between donor(SOD) and acceptor(MeJA) was obtained according to Frster theory of non-radioactive energy transfer.