ISOLATION AND CHARACTERIZATION CYTOTOXIC COMPOUNDS CAFFEINE AND CHLOROGENIC ACID SEEDS OF LAMPUNG COFFEE ROBUSTA
2013
Therapeutic use of natural compounds as the active ingredient is a breakthrough anticancer ingredient in pharmaceuticals and medicine. Caffeine and chlorogenic acid have isolated from coffee, there are cytotoxic and contain high antioxidants. Objective: to determine cytotoxic and antioxidants effect of caffeine and chlorogenic acid in coffee rubusta Lampung. Methods: Research have carried out by several steps including: separation and purification via extraction and fermentation of robusta coffee beans, maceration using methanol solvent, fractionation using n-hexane and water, the purification is done by using various combinations of chromatographic techniques using normal phase and reversed-phase column on an open and press column, the purity of the active compound analysis by performed the method of thin layer chromatography (TLC) and reversed and normal phase high performance liquid chromatography method (HPLC / HPLC), toxicity tests carried out on brine shrimp (A. salina), and the antioxidant test using the method trapping of free radicals 1.1 Diphenyl-2-picrylhidrazyl (DPPH). Result: Isolates of caffeine has cytotoxic activity against larvae of shrimp with a value of 100% mortality at 1000 ppm, while the smallest 60% mortality at concentrations of 10 ppm with IC50 values for caffeine obtained at 21.41 ppm. Whereas chlorogenic acid has a cytotoxic activity with a value of 100% mortality at concentrations of 100, 300, and 1000 ppm and the smallest 70% mortality at concentrations of 10 ppm with IC50 values of 5.86 ppm. Conclusion: Both compounds have a cytotoxic activity with LC50 values of less than 1000 ppm, and chlorogenic acid had higher antioxidant activity compared to caffeine.
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