Food restriction reduces hepatic alterations associated with experimental periodontitis.

BACKGROUND Periodontitis is a chronic inflammatory and multifactorial disease that affects the periodontal structures and can cause alterations in the hepatic tissue. The aim of the present study was to evaluate whether a diet with food restriction can decrease oral and liver alterations associated with ligature-induced periodontitis. METHODS Twenty-four female Wistar rats were used in this study, randomized into three groups (n = 8 for each group): control (regular food); periodontitis (regular food + periodontitis induced with ligatures) and food restriction (diet with food restriction and periodontitis induction). The following periodontium parameters were analyzed tooth mobility (TM), probing pocket depth (PPD), gingival bleeding index (GBI) and alveolar bone height (ABH). In the liver, the levels of oxidative stress marker - malondialdehyde (MDA), glutathione (GSH), total cholesterol and levels of myeloperoxidase (MPO) activity were measured. Liver samples were analyzed for histopathological score. In the blood tissue, the levels of enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose, total cholesterol and the high-density lipoprotein (HDL) were also evaluated. RESULTS The animals that received a diet with food restriction + periodontitis showed a decrease in hepatic histopathological score (p<0.05) when compared to the periodontitis group, the same for glucose, total cholesterol, ALT, AST and ABH data. The group with food restriction + periodontitis showed a decrease in the histopathological liver score (p <0.05) compared to the group with periodontitis. CONCLUSION This study revealed that food restriction reduced oral damages, as well as hepatic, blood and alveolar bone alterations associated with ligature-induced periodontitis in rats. This article is protected by copyright. All rights reserved.