Immunohistochemistry: A Method to Analyze M. ulcerans Infected Skin Tissue.

Immunohistochemistry (IHC) is a combination of immunological, biochemical, and pathological methods to visualize the presence and distribution of specific epitopes in tissue sections. Selected antigens are stained by differently labeled antibodies binding to their target antigens in situ. Here we describe sample preparation and sample staining in order to diagnose and analyze tissue samples infected with M. ulcerans from human as well as animal source.