[Wogonin inhibits IGF-1-stimulated cell growth and estrogen receptor α expression in breast adenocarcinoma cell and angiogenesis of chick chorioallantoic membrane].

The aim of the present study was to investigate the involvements of insulin-like growth factor-1 (IGF-1) and estrogen re-ceptor α (ERα) in the inhibitory effect of wogonin on the breast adenocarcinoma growth.Moreover,the effect of wogonin on the an-giogenesis of chick chorioallantoic membrane (CAM) was also investigated.MCF-7 cells (human breast adenocarcinoma cell line) were subjected to several drugs,including IGF-1,wogonin and ER inhibitor ICI182780,alone or in combination.MTT assay was used to detect breast cancer proliferation.Western blot was used to analyze ERα and p-Akt expression levels.CAM models prepared from 6-day chicken eggs were employed to evaluate angiogenesis inhibition.The results showed wogonin and ICI182780 both exhibited a potent ability to blunt IGF-1-stimulated MCF-7 cell growth.Either of wogonin and ICI182780 significantly inhibited ERα and p-Akt expressions in IGF-1-treated cells.The inhibitory effect of wogonin showed no difference from that of ICI182780 on IGF-1-stimulat-ed expressions of ERα and p-Akt.Meanwhile,wogonin at different concentrations showed significant inhibitory effect on CAM an-giogenesis.These results suggest the inhibitory effect of wogonin on breast adenocarcinoma growth via inhibiting IGF-1-mediated PI3K-Akt pathway and regulating ERα expression.Furthermore,wogonin has a strong anti-angiogenic effect on CAM model.