Effect of benzoic acid hydroxy- and methoxy- ring substituents during cucumber (Cucumis sativus L.) germination. I.: Isocitrate lyase and catalase activity

Abstract Benzoic acids (BAs) are released by plants through leaching of leaf exudates and rhizodeposition. Most of them exert allelopathic effects. BAOH ring substituents generally caused an increase in germination percentage, whereas OCH 3 ring substituents decreased the percentage of germination. Hydroxy substituents increased isocitrate lyase (ICL) activity, whereas OCH 3 ring substituents decreased enzyme activity indicating a direct effect on enzyme catalysis. The addition of BAs to the reaction mixture containing extracts from seedlings incubated with the related BA derivatives and to extracts from controls confirmed the direct stimulating (OH) and inhibiting (OCH 3 ) effect of BA substituents on ICL catalysis. Catalase (CAT) activity was dramatically increased by hydroxylated BAs, even though some methoxylated BAs exerted a strong activating effect. Native and SDS-PAGE analyses indicate the presence of two bands of 236 and 66 kDa, which correspond to ICL enzyme activity levels. The enhancing of silver staining and the disappearance of these bands are related to ICL enzyme activity suggesting a possible effect of BAs on synthesis of ICL. The physiological effect of BAs on cucumber germination is also discussed.