Activated CD4+ T Cells Target Mesangial Antigens and Initiate Glomerulonephritis
2012
Aims—The role of kidney infiltrating T cells in the pathology of lupus nephritis is unclear. This study was undertaken to investigate whether CD4+ T cell responses to a surrogate mesangial antigen can initiate glomerulonephritis. Methods—Ovalbumin was deposited in the glomerular mesangium of C57BL/6 (B6) mice using anti-α8 integrin immunoliposomes (α8ILs). This was followed by injection of activated ovalbumin-reactive CD4+ transgenic OT2 T cells. Trafficking of antigen-specific OT2 T cells to kidneys and lymph nodes was studied by flow cytometry. Glomerular pathology and immune cell infiltration was characterized by immunostaining. Role of CCR2 deficiency on T cell mediated glomerulonephritis was investigated using B6.ccr2 −/− mice. Results—α8ILs delivered ovalbumin specifically to the renal glomeruli. Adoptively transferred OT2 T cells preferentially accumulated in renal lymph nodes and in the renal cortex. Kidneys showed glomerular inflammation with recruitment of endogenous T cells, dendritic cells and macrophages. T cell mediated inflammation induced mesangial cell activation and an increase in glomerular MCP1 and fibronectin. The formation of inflammatory foci was driven by Ly6C monocytes and was CCR2 dependent. Conclusions—The findings from this study show that T cells reactive with antigens in the mesangium are sufficient to initiate glomerular pathology. Antigen-specific CD4 T cells act by inducing glomerular MCP1 production which mediates recruitment of inflammatory monocytes resulting in glomerulonephritis. Thus, down modulation of T cell responses within the kidneys of lupus patients will be a beneficial therapeutic approach.
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