Comparison of DIPs and STRs detection in human remains

Diallelic deletion/insertion polymorphisms (DIPs) detection and short tandem repeats (STRs) analysis were compared using Investigator® DIPplex kit (Qiagen, Germany) and AmpFISTR®Identifiler®Plus kit (Applied Biosystems, USA). A total of seven human remains samples (two bone, one tooth and four tissue samples) were isolated using organic extraction and quantified by Quantifiler® Human DNA Quantification kit (Applied Biosystems, USA), and amplified with above mentioned amplification kits. Capillary electroforesis was performed on 3130xl Genetic Analyzer instrument (Applied Biosystems, USA).PCR amplification results for six samples (two bone and four tissue samples) using Investigator® DIPplex kit provided better results when compared to AmpFISTR®Identifiler®Plus kit . In two bone samples and one tissue sample full DNA profile was obtained using Investigator® DIPplex kit, while amplification using AmpFISTR®Identifiler®Plus kit resulted in partial DNA profile or complete loss of DNA profile. In three tissue samples full DNA profiles were identified using Investigator® DIPplex kit in duplicate, while partial DNA profile was detected only once. Amplification of three tissue samples using AmpFISTR®Identifiler®Plus kit resulted in partial DNA profile or complete loss of DNA profile. PCR amplification of one tooth sample using both kits resulted in full DNA profile.