THE EFFECT OF THE CIGARETTE SMOKE ON ACTIVITIES OF SOME SERUM ENZYMES IN DIABETIC RATS

Although Nicotine has been known as the most toxic component of cigarette, many of the effects of smoking, such as cardiovascular disease, respiratory disease, cancers, especially lung, throat and mouth cancer are not related to the nicotine, but are related to the other toxins and carcinogens in the smoke or extract of tobacco. Cigarette smoke contains the main and lateral (active and passive) smokes. Lateral smoke is the smoke from a burning cigarette, which is inhaled by people around the smoker and the smoke is much more toxic than mainstream cigarette smoke. According to studies, it has been shown that cigarette smoke has a great influence on the mandatory or voluntary companions of the smokers. In this study the effects of passive smoke inhalation on the activity of serum enzymes in diabetic rats were evaluated. In this intervention study, 24 male Wistar rats weighing 200 to 220 g randomly divided into 4 groups of 6 animals: healthy control, diabetic control, smoker treatment and smoker-diabetic treatment groups. In the diabetic control and treatment groups the diabetes was induced with a single dose (65mg / kg) injection of streptozotocin in the IP form. The blood sugar levels of rats were measured using a glucometer after 24 hours; so, the rats with higher glucose levels than 250 mg / dl were considered as diabetic. In healthy control group no intervention was applied. The healthy treatment and diabetic treatment groups were exposed to the inhalation of passive smoke of a cigarette burned during 1 to 2 minutes for 30 days routinely. After one month, the rats were decapitated and the blood samples were obtained. The samples were centrifuged and the serum was separated. The activity of serum AST, ALT, ALP, LDH and CPK were measured using diagnostic kits and spectrophotometric method and the obtained results were analyzed by ANOVA method. During the study, all groups were exposed to the same environmental and light conditions as well as unlimited access to food and water. Statistical comparison of the results obtained in this study showed that there is no significant difference in understudied factors of the groups.