Curcuminoids in Zingiber zerumbet Rhizomes: Bioguided Fractionation and Chromatographic Identification of Antimicrobial and Antioxidant Metabolites

ABSTRACTMetabolite extraction from Zingiber zerumbet rhizomes using solvents of varying polarity index revealed high antimicrobial activity in the isopropanol fraction, whereas higher free-radical scavenging and antioxidant activity were observed in the benzene extract, which also had higher total phenolics and tannins. Chromatographic and spectral analysis of the benzene fraction revealed curcuminoids as the major active component. Fourier transform infrared (FTIR) spectrometry confirmed the presence of alkanes, carboxylic acids, amides, ketones, and aldehydes in the bioactive solvent extract.