Cloning and Sequential Analysis of the Cytokine from Mouse

To analyze the diversity of expression quantity and lay foundation for real time fluorescent quantitation PCR, spleen samples of mouse were collected and mRNA were abstracted, furthermore, the mRNA were reverse transcribed into cDNA by using kit. Six cytokine genes IL-6, IL-12a, IL-12b, IFN-γ, TNF-αand iNOS were cloned and sequenced. The results demonstrated that the homogeneities of the sequences between amplified genes and cytokine genes in genebank all reached above 99%.