BIO-POTENTIATION AND IN VIVO PHARMACOKINETICS STUDIES OF POLYPHENOLS FROM PUNICA GRANATUM BY FORMING PHOSPHOLIPID CONJUGATES FOR BETTER ANTIOXIDANT & ANTI-INFLAMMATORY ACTIVITY

Objective: Polyphenols belong to the chemical class of flavonoids and are widely distributed in vegetables and plants. In this study, we aimed to potentiate the antioxidant and anti-inflammatory effects of polyphenols by forming a phospholipid complex of extracts from Punica granatum and also evaluating its in vivo pharmacokinetic parameters. Methods: This method was used for determination and in vivo pharmacokinetics studies for the biomarkers and their lipid complexes from P. granatum extract. Anti-inflammatory and antioxidant studies ever performed on both uncomplexed and complexed flavonoids. Pharmacokineticbehaviour of biomarker was significantly improved with higher C max , t 1/2 and shorter t max & AUC 0-t and AUC 0-t values of glucuronide metabolites indicating the greater bioavailability. Results: Anti lipid peroxidation inhibitory potential was found greater in case of phospholipid complexes with IC 50 value 17.41 µg for biomarker complex as compared to biomarker mixture (63.52 µg) & anti-inflammatory activity of 100 mg/kg of agro waste extract complex of P. granatum was found very significant at 3rd hour with (21.95±0.071 %) inhibition in rat paw edema which was greater than the extract alone (11.13±0.084 %). Conclusion: Bio-enhancement of polyphenols was observed and achieved through phospholipids complexes and was demonstrated by in vitro and in vivo activity testing by forming a supramolecular complex.