Expression of Maspin, KI-67 and CD105 as Predictors of Postoperative Recurrence in Laryngeal Carcinoma: perioperative planning and proposed reconstructive tools

Background: Maspin, a member of serpin super-family, has multifaceted biological functions and an unique tumor suppressing activity. Several studies showed that maspin suppresses tumor growth, angiogenesis, invasion and metastasis. The present study investigated the relationship between maspin expression, Ki-67 proliferative index (PI), CD105-assessed microvessel density (MVD) and postoperative recurrence in laryngeal squamous cell carcinoma (SCC). Patients and methods: Subcellular pattern of maspin expression was immunohistochemically evaluated in 28 cases of laryngeal SCC treated by total laryngectomy with reconstruction but without primary radiotherapy with a follow-up period from 10 to 36 months. The expression and interaction between Ki-67, CD105 and maspin were also studied. Results: Two patterns of positive maspin expression; cytoplasmic (n=9) and nuclear-cytoplasmic (n=16) were recognized. Significant inverse correlation between nuclear-cytoplasmic pattern and both Ki-67 PI (P=0.049) and CD105-assessed MVD (P=0.016) were disclosed. Comparing the two groups of patients with (pR+) and without (pR-) evidence of postoperative recurrence, none of the studied clinicopathological parameters (age, sex, pathological grade, tumor stage, and nodal stage) was significantly associated with recurrence (all p>0.05). The nuclear-cytoplasmic maspin expression was significantly higher in pR- patients (p=0.018), while higher Ki-67 PI and CD105-assesed MVD were significantly correlated with pR+ group (p= 0.007 & p = 0.004 respectively). Conclusion: The present results suggest that absence of nuclear localization of maspin and high Ki-67 PI and CD105-assessed MVD may predict a higher risk of recurrence in laryngeal SCC patients. (Mona G. Shafeek, Mona M. EL-Sayed, Mohammad R. Ahmad and Wail Fayez Nasr. Expression of Maspin, KI- 67 and CD105 as Predictors of Postoperative Recurrence in Laryngeal Carcinoma: perioperative planning and proposed reconstructive tools. Journal of American Science 2011;7(3):476-484). (ISSN: 1545-1003). http://www.americanscience.org.