A method and kit for the determination of DNA double / single-strand breaks and apparatus for controlled filtering of particular DNA molecules and / or other molecules / molecular complexes containing liquid media with well filter plates

1999 
A method for the determination of DNA double-strand breaks with the following steps: a) task of to be examined cells and / or tissues at specific filters. b.) lysis of the cells / tissues with at least one detergent, at least one chaotropic substance, at least one metal ion complexing and DNA repair enzymes inhibiting substance and at least one specific double-stranded DNA binding fluorescent dye, or lysis of cells / tissues with at least one detergent, at least a chaotropic substance, at least one protease and at least one double-strand-specific DNA-binding fluorescent dye, c) elution of the released by the lysis DNA fragments by the specific filter. d.) performing a fluorescence spectroscopic analysis, only one of eluate fraction is collected per well, or a) task of to be examined cells and / or tissues at specific filters. b.) lysis of the cells / tissues with at least one detergent, at least one chaotropic substance and at least one metal ion complexing and DNA repair enzymes inhibiting substance, or Lysis of cells / tissues with at least one detergent, at least one chaotropic substance and at least one protease, ...
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