Determination of circulating immune complexes by immunoenzyme method

: Enzyme immunoassay is proposed to be used for measuring the content of circulating immune complexes (CIC) in human blood serum. S. aureus protein in a concentration of 1 to 5 micrograms/ml optimal for the sensitization of plates is used as the first sandwich layer. The method permits registration of CICs formed by immunoglobulins of different classes. Investigations of the blood sera of donors and cancer patients by the proposed method and by other methods (V. Haskova et al. and M. Digeon et al.) to assess complex formation showed a good correlation of the results of all measurements.