Régénération de vitroplants par embryogenèse somatique à partir d'embryons zygotiques immatures chez une espèce adaptée à la sécheresse: Acacia tortilis subsp. raddiana (Savi.) Brenan

Abstract Using calli from immature zygotic embryos of Acacia tortilis subsp. raddiana, globular somatic embryos are obtained after 90 to 100 days culture in darkness on VA media added with 2,4-D (2,4 and 8 mg/l) alone or in combination with BAP (1 and 0,5 mg/l). Light and transfert on MS/2 medium containing BAP (0,5 mg/l)) and AIB (0,05 mg/l) enhance proliferation of somatic embryos. ABA (2,6 and 5,2 mg/l) or high concentration of sucrose (50 g/l) allow development from globular to bipolar embryos. Development of mature somatic embryos into plantlets are obtained on MS/2 medium without hormon, but supplemented with high concentration of sucrose.