SuperhydrophobicGlass Microfiber Filter as Background-FreeSubstrate for Quantitative Fluorometric Assays
2020
We have discovered herein that commonly
used laboratory glass microfiber
filters can be functionalized as background-free superhydrophobic
substrates for quantitative fluorometric assays. In particular, glass
microfiber filters (Whatman GF/A) can be treated with low-concentration
(20 mM) methyltrichlorosilane/toluene solution to be superhydrophobic
(water contact angle >150°) in less than 5 min; the modified
glass microfiber filter can be readily patterned with UV/ozone irradiation
to create hydrophilic reaction zones on the otherwise superhydrophobic
substrate. Compared with traditional cellulose filter paper, the glass
microfiber filter has extremely low fluorescence background, which
makes it an excellent substrate for preparing quantitative fluorometric
assays. In conjunction with smartphone imaging and color analysis,
we have showcased a copper(I)-catalyzed azide-alkyne cycloaddition
(CuAAC)-based fluorometric assay for copper quantitation on these
patterned, superhydrophobic glass microfiber filter substrates. Both
the limit of detection and linear response range are comparable with
the standard spectrophotometric quantitation in solution and commercial
copper detection kits, which augments the application potential of
superhydrophobic glass microfiber filters as ideal (e.g., background-free)
substrates for the preparation of multiplex microassays and other
advanced microanalytical devices based on fluorescence readout.
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