Defining the ionic mechanisms of optogenetic control of vascular tone by channelrhodopsin-2.

Figure S1 Effects of [KCl]e on the tension of rings of various artery types isolated from ChR2(H134R)‐SM mice. Mean tension versus [KCl]e relationships for the different artery types (aortic, A, (N=20, n=32); mesenteric, B, (N=5, n=11) and pulmonary, C, (N=5, n=11)). The curves through the filled symbols represent the best fit of the data with Eq. 5. Figure S2 CaV channel currents in isolated aortic VSMCs A. Representative whole‐cell CaV channel currents obtained from aortic VSMCs isolated from ChR2(H134R)‐SM mice in response to the stimulation protocol shown in the top panel. For clarity, only the currents elicited every 20 mV are displayed. B. Mean steady‐state activation (N=8, n=15) and inactivation (N=7, n=12) curves for CaV channels measured in aortic VSMCs. Smooth curves represent the fit of the data using Eq. 3 and 4 (activation and inactivation, respectively). Grey bar highlights the range of V m that intact VSMCs can experience as a result of ChR2(H134R)‐mediated depolarisations. C. Mean whole‐cell CaV currents versus V m relationships in the absence (N=8, n=15) or presence of 1 μM nifedipine (Nif) which was either kept in the dark (N=5, n=9) or exposed to blue light (2.4 mW/mm2) for 10 min (light conditioned) (N=6, n=20), as indicated. Curves through symbols represent the best fit of the data with Eq. 3. Figure S3 Effects of noradrenaline on the tension of aortic rings isolated from ChR2(H134R)‐SM mice. Mean tension versus noradrenaline concentration ([NA]) relationship (N=5, n=8). The curve through the filled symbols represent the best fit of the data with Eq. 5. Table S1 Parameters for tension versus [KCl]e relationships for artery rings obtained from ChR2(H134R)‐SM mice. Table S2 Parameters for tension versus [NA] relationships for artery rings obtained from ChR2(H134R)‐SM mice. Click here for additional data file.(472K, pdf)