Global quantitative proteomic analysis profiles host protein expression in response to Sendai virus infection

Sendai virus is an enveloped non-segmented negative-strand RNA virus that belongs to the genus Respirovirus of the Paramyxoviridae family. As a model pathogen, SeV has been extensively studied to define the basic biochemical and molecular biologic properties of the paramyxoviruses. In addition, SeV-infected host cells were widely employed to uncover the mechanism of innate immune response. To identify proteins involved in the SeV infection process or the SeV-induced innate immune response process, system-wide evaluations of SeV-host interactions have been performed. cDNA microarray, siRNA screening and phospho-proteomic analysis suggested that multiple signaling pathways are involved in SeV infection process. Here, to study SeV-host interaction, a global quantitative proteomic analysis was performed on SeV-infected HEK 293T cells. A total of 4,699 host proteins were quantified, with 742 proteins being differentially regulated. Bioinformatics analysis indicated regulated proteins were mainly involved in “IFN-I signaling pathway” and “defense response to virus”, suggesting these processes play roles in SeV infection. Further RNAi-based functional studies indicated that the regulated protein TRIM24 and TRIM27 affect SeV induced IFN-I production. Our data provided a comprehensive view of host cell response to SeV and identified host proteins involved in the SeV infection process or the SeV-induced innate immune response process. This article is protected by copyright. All rights reserved