HIV-1 ADA株gp140真核表达载体的构建与鉴定

Objective To construct an expression plasmid bearing the gp140 envelope glycoprotein (env) gene of HIV-1 ADA strain. Methods Two pairs of primers for ADAgp140 gene were designed and overlap PCR was taken placed. Recombinant plasmid pcDNA6-ADAgp140 was constructed by ligating plasmid pcDNA6/myc-HisA and the PCR product. The recombinant plasmid was identified with PCR and DNA sequencing. Results PCR product was obtained and inserted into plasmid pcDNA6-ADAgp140. The recombinant plasmid was transformed into E. coli. Sequencing result showed that the modified env had correct nucleotide sequence. Conclusion Expression plasmid for HIV-1 ADA env gp140 is constructed successfully. It provides possibility for further study on the biological characteristics of HIV envelope glycoprotein and vaccine.