Protecting effects of topical hypothermia to blood-ocular barriers in intraocular operation

Objective:To investigate whether topical hypothermia circumstance has the protective effects to the blood-ocular barriers during intraocular operations.Methods:Twenty New Zealand albino rabbits accepted vitrectomy in both eyes,useing hypothermal(4-10℃) intraocular irrigating solution in left eye(hypothermal intraocular irrigating solution group),and useing room temperature(25℃) intraocular irrigating solution in right eye(room temperature intraocular irrigating solution group)during the vitrectomy.Twenty-four hours after the operation,the retina of both eyes were checked;each eye accepted cavum vitreum perforation,and 0.5 ml of intraocular fluid were aspirsted for biochemical detection as well;as well as the retinas cut off for transmission electron microscopical(TEM) observations. Results:Inflammatory reaction of anterior ocular and the uvea in left eyes were generally slighter than the right eyes;dioptric media clarity of left eyes were also better than that in left eyes;average total protein concentration of the intraocular fluid was obviously lower than that in left eye(P0.01);Viewed with TEM,retinal ganglionic cells of left eyes showed milder degenerative changes,cells organ slightly swelled and maintained their basic shapes,but retinal ganglionic cells of right eyes were highly swelled and where in various states of significant degeneration.Conclusion: In laboratory animal intraocular operation,topical hypothermia circumstance can reduce the inflammatory reaction of operation trauma,and this can propect blood-retina barrier.