Nutrient effects on growth and armentomycin production in cultures of Streptomyces atmentosus
1995
A high-performance liquid chromatographic method for the analysis of armentomycin (L-2-amino-4,4-dichlorobutanoic acid) in culture broths was developed and calibrated using DL-2-amino-4,4-dichlorobutanoic acid synthesized in five steps from methyl acrylate, carbon tetrachloride, and sodium azide. Armentomycin was produced optimally (5–10 mM during 8 days' fermentation) by Streptomyces armentosus in media containing starch, lysine, chloride ions, and a low concentration of phosphate. Production was markedly affected by carbon and nitrogen sources: of those tested, starch supported both the highest biomass yield and the highest armentomycin titre; lysine was the best source of nitrogen for growth and for achieving the maximal armentomycin titre. Increasing the concentration of lysine from 40 to 120 mM nitrogen increased armentomycin production, and gave no evidence of nitrogen catabolite repression. Growth and armentomycin biosynthesis increased as the initial concentration of phosphate in media was increas...
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