Distribution and Resistance Analysis of Isolates from Blood Culture Samples

OBJECTIVE To investigate the distribution and resistance of pathogenic bacteria isolated from the blood culture specimens collected during the period of Sept 2007 Dec 2008.METHODS The 5045 blood cultures were detected by BacT/Alert 3D automated blood culture system,and bacterial susceptibility test was conducted on all isolates using Kirby-Bauer methods with CLSI standards.WHONET 5.4 was applied for analysis of the bacterial sensitive data.RESULTS From the 5045 blood samples received,we identified 408 strains(8.1%) of pathogens in which 193 Gram-positive strains(47.3%),197 Gram-negative strains(48.3%),17 fungal strains(4.2%),and 1 anaerobes strain(0.2%).Coagulase-negative staphylococcus were 121 strains(29.7%),Escherichia coli 76 strains(18.6%),Enterococcus 36 strains(8.8%),Klebsiella 29 strains(7.1%),and Staphylococcus aureus 18 strains(4.4%).The main pathogenic bacteria alarmed were Enterobacteriaceae,particularly was E.coli in 12 hours,Enterobacteriaceae and Enterococcus in 12-18 hours,Staphylococcus in 18-24 hours,and Staphylococcus and fungi after 24 hours.No Staphylococcus strain was resistant to vancomycin.No Enterobacteriaceae strain was resistant to carbapenems.Some isolates of E.faecium showed resistance to vancomycin and teicoplanin.CONCLUSIONS It is very important to obtain the results of blood culture in time for correct clinical therapy of bacterial infective diseases and to improve the cure rate and to control cross-infection in hospitals.