Chrysanthemum phloem necrosis: detection by epifluorescence microscopy

In epifluorescence diagnostic procedures for chrysanthemum phloem necrosis, autofluorescence, aniline blue, and various nucleic acid "specific" fluorochromes such as 33258-Hoechst, 4′,6-diamidino-2-phenyl-indole-dichloride, berberine sulfate, ethidium bromide, and acridine orange were utilized. Increased fluorescence in foliar phloem correlated precisely with gross symptoms of chrysanthemum phloem necrosis by all techniques and was used, in the case of berberine sulfate, to facilitate detection of mycoplasmalike organisms by transmission electron microscopy.