Ion-pair liquid chromatographic assay of angiotensin-converting enzyme activity

Abstract A rapid and specific high-performance liquid chromatographic assay for the quantitative determination of angiotensin-converting enzyme activity is described. Hippuryl- l -histidyl- l -leucine (Hip—His—Leu) is used as substrate and the released hippuric acid is measured. The procedure is accurate and precise and no extraction is required.