Deteksi polimorfisme gen growth hormone (GH) pada sapi Sumba Ongole (SO)

Anwar S, Agung PP, Wulandari AS, Sudiro A, Said S, Tappa B. 2015. Detection of growth hormone (GH-MspI) gene polymorphism in Sumba Ongole (SO) cattle. Pros Sem Nas Masy Biodiv Indon 1: 398-403. Detection of gene polymorphisms was important to get early information in the identification of genetic markers associated with economically desirable traits. Growth hormone gene has been used as candidate genetic marker related to growth traits in beef cattle. Sumba Ongole (SO) cattle (Bos indicus) originated from Sumba Island, East Nusa Tenggara, Indonesia which has potential genetic as superior local beef cattle breed. This study was aimed to detect GH-MspI gene polymorphism in SO cattle. GH-MspI gene polymorphism was detected using PCR-RLFP method and followed by sequencing analysis. Growth hormone gene sequence from GenBank (EF592534) was used as a reference for this study. A specific fragment of GH gene (1072 base pairs) was successfully amplified with the annealing temperature at 57°C. The amplified DNA fragments were digested using MspI restriction enzyme and visualized by gel electrophoresis for genotype identification. Polymorphism of GH-MspI gene in SO cattle has been detected with the presence of three variants of restriction pattern of DNA. The sequence alignment analysis has been successfully detected in the two point mutations. The first was a nucleotide substitution in the third intron (1047T>C) and the second was a nucleotide insertion in the fourth intron (1395_1396insC). The first and second point mutations were found revealed that there was GH gene sequence variations within and between cattle breed, respectively.