Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase(YCJ01)

In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization(ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase(YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length(weight gain 3200%) was, the higher the activity of immobilized lipase was.