Construction of eukaryotic expression plasmids coding BoIL-18 and its immunoenhancement on Foot-and-Mouth Disease virus vaccine.

【Objective】 The research was to ascertain the immunoenhancement effect on vaccine.【Method】 The coding region sequence of complete BoIL-18 gene was first inserted into pcDNA3.1 vector,resulting in the construction of IL-18 gene eukaryotic expression plasmids(pcDNA3.1-BoIL18).Immunoenhancement of the pcDNA3-BoIL18 plasmid on Foot-and-Mouth Disease(FMD)Virus Vaccine was observed by the test of neutralizing antibody,proliferation response of the T lymphocytes from spleen,and the cytotoxicity of NK cells.At the same time,the pcDNA3.1-BoIL18 plasmid was distinguished from empty pcDNA3.1 vector and FMD virus vaccine only.【Result】 Proliferation responses of T and the cytotoxicity of NK cells were stronger than those of control group after 21st day plasmids and vaccines were significantly higher than those of mice only inoculated with vaccines after 28th day inoculation.But the mice inoculated with empty pcDNA3.1 vector and vaccines and vaccines only were not discrepant.【Conclusion】 BoIL-18 has obvious immunoenhancement effects on Foot-and-Mouth Disease Virus Vaccine.