In Vitro Observations on the Influence of Copper Peptide Aids for the LED Photoirradiation of Fibroblast Collagen Synthesis

Objective: The purpose of this study was to evaluate the influence of Cu-GHK aids for the LED-PI on fibroblast proliferation and collagen production in vitro. Background Data: Light-emitting diode photoirradiation (LED-PI) and copper-glycyl-L-histidyl-L-lysine complex (Cu-GHK) treatment may be useful in accelerating the rate of wound healing. Red LED (625–635 nm) was used as a light source for LED-PI. In the process of wound healing, Cu-GHK was shown to be an activator of remodeling. LED-PI would maintain fibroblast activity and viability, and there would be a positive effect on type I collagen (COL1) and basic fibroblast growth factor (bFGF) production from the combination of LED-PI and Cu-GHK incorporation. Methods: Cell activity/viability, procollagen type I C-peptide (P1CP), and bFGF were evaluated in vitro with human fibroblasts (HS68). The effects of single factors (LED-PI using 0, 1, and 2 J energy doses) or a combination of factors (LED-PI and Cu-GHK) on fibroblast viability (i.e., alamarBlue™ red...