Gene Expression Change Related Inflammation Pathway during Rat EVLP and Heat Stress

Purpose Although Ex Vivo lung perfusion (EVLP) is a useful technique for evaluating and repairing the donor lung in lung transplantation, it can cause various lung injuries. In this study, we investigated the inflammation pathways that induces lung injury during EVLP through RNA sequencing. And, we examined how perfusate temperature during EVLP changes the inflammatory pathways. Methods Fifteen male Sprague Dawley rats were randomly divided into a sham group(n=5), normothermic EVLP (37°C,n=5) and hyperthermic group (42°C, n=5). In hyperthermic group, 5 minutes of heat stress (42°C) was given during 4 hours EVLP. Transcriptome analysis was performed with lung tissues of sham (n=2), normothermic EVLP (n=2), and hyperthermic EVLP (n=2). Results A total of 7172 genes were significantly (P Conclusion In this study, we identified inflammation pathways that causes lung injuries during EVLP at gene level using RNA sequencing. This technique could be useful to select proper treatments for lung injury during EVLP by present the injury pathway corresponding to a specific temperature of perfusate.