IN-VITRO ANTI-OXIDANT AND ANTI-DIABETIC POTENTIAL OF METHANOLIC EXTRACT OF AGARICUS BISPORUS (WHITE BUTTON) ANDCOPRINUS COMATUS (SHAGGY MAN) -

Diabetes mellitus is considered, the sixth main cause of death throughout the world, and every ten seconds a person dies from causes related to diabetes, mainly due to cardiovascular complications. Mushrooms have been used for traditional foods and medicines globally. The current study was tried to evaluate the in-vitro anti-oxidant and antidiabetic properties of methanolic extracts of the Agaricus biosporus and coprinus comatus using alpha (α)-amylase, beta (β)-glucosidase assays and anti-oxidant efficacy by applying ABTS and DPPH radical scavenging assays. The Agaricus biosporus methanolic extracts (ABME) showed significant inhibitory effects of DPPH (0.48± 0.002) and ABTS (0.11 ±0.01) and C.comatus DPPH (2.50±0.12) ABT (3.16±1.4) with an IC50 value respectively as compare with standard drug ascorbic acid. The selected mushrooms species methanolic extracts exhibited significant inhibitory activities of β-glucosidase and α-amylase having IC50 value at a concentration of 0.75μg , 0.80μg extracts in the same way as compared with the standard drug Glucophage. Our results suggest that the Agaricus biosporus and coprinus comatus mushroom could be useful for the control of diabetes and free radicals produced by different metabolic activities in our body.