XRCC1 Arg194Trp and Arg399Gln polymorphisms and lung cancer risk in Romanian population

Introduction X-ray repair cross-complementing 1 (XRCC1) protein has an specific funtion in base excision repair. Single nucleotide polymorphisms (SNPs) in XRCC1 gene may influence DNA repair ability and thus modulate cancer susceptibility. The aim of our study was to investigate the association between XRCC1 Arg194Trp and Arg399Gln polymorphisms and lung cancer risk, in Romanian population. Material and method. We performed a case–control study to test the association between lung cancer risk and two polymorphisms of the XRCC1 gene (Arg194Trp and Arg399Gln). We recruited 102 subjects with lung cancer and 122 controls. Genotyping was performed by multiplex PCR-RFLP. Results. Comparative analysis to assess the risk for lung cancer in the study group compared with the control group for variant allele XRCC1 gene 194Trp carriers (OR dominant model), identifies a risk at the limit of statistical significance (χ2 = 0.135,p = 0.052, OR = 2.675 CI = 0.963 to 7.432). Also, statistical comparative analysis (OR recessive model), revealed an increased risk for lung cancer, for homozygous 194Trp genotype (χ2 = 0.186 p = 0.007, OR = 10.667 CI = 1.309 - 86,933). Comparative analysis to assess the risk for lung cancer risk in the study group compared with the control group for the variant Gln399Arg XRCC1 gene variant carriers (OR dominant and recessive models), does not identify an increased risk for lung cancer (χ2 = 0.036 p = 0.606, OR = 1.160, CI = 0.660 to 2.037; χ2 = 0.044 p = 0.526, OR = 1.276 CI = 0.600 to 2.716). Conclusions. The results of the study place the XRCC1 gene Arg194Trp polymorphisms among independent risk factors for lung cancer development.