Assessment of the in vitro gamma-H2AX assay by High Content Screening (HCS) as a novel genotoxicity test: application for the evaluation of single toxicants and mixtures

Figure 2: γH2AX frequency (solid lines) and RCC (dashed lines) after 3 and 24 hr exposure to [A] direct acting agent ethyl methanesulfonate; [B] ROS generator bleomycin sulfate; [C] topoisomerase inhibitor etoposide. RCC error bars (n=3) are not included in the graphs for clarity. * Indicates minimum concentration showing a significant increase in γH2AX frequency compared to the vehicle-treated control.