Thidiazuron Promotes In Vitro Plant Regeneration of Arachis correntina (Leguminosae) via Organogenesis

Arachis correntina (Burkart) Krapov. & W.C. Gregory is a herbaceous perennial leguminous plant growing in the Northeast of the Province Corrientes, Argentina. It is important as forage. The development of new A. correntina cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to investigate the plant regeneration potential of mature leaves of A. correntina in tissue culture. Buds were induced from both petiole and laminae on 0.7% agar-solidified medium containing 3% sucrose, salts and vitamins from Murashige and Skoog (MS) supplemented with 0.5–25 μM thidiazuron (TDZ). Shoot induction was achieved by transference of calli with buds to MS supplemented with 5 μM TDZ. Fifty-four percent of the regenerated shoot rooted on MS + 5 μM naphthaleneacetic acid. Histological studies revealed that shoots regenerated via organogenesis.