Erratum to “GLUT14, a duplicon of GLUT3, is specifically expressed in testis as alternative splice forms”

Fig. 1. (B) Expression patterns of GLUT3 and GLUT14. Three forward primers crossing exons 1/2 and reverse primer on exon 7 were used for RT-PCR against total RNAs from various human tissues. The PCR products were confirmed to be GLUT3 or GLUT14 cDNA by sequencing. Fig. 3. Expression of GLUT3 and GLUT14 in testis. (A) cDNA-specific primers, forward primers crossing exons 1/2 and reverse primer on exon 7 (see text for sequences), were used for RT-PCR against total RNAs from human testis. Each PCR was performed with the same conditions except for the cDNA-specific forward primers. PCR products were confirmed to be GLUT3 or GLUT14 cDNA by sequencing. (B) Northern blot of GLUT3 and GLUT14L. Human testis total RNA 10 g (Clontech) each lane was separated with agarose/formaldehyde gel and transferred to membrane. The probes labeled with digoxigenin were generated by PCR with cDNA-specific primers against GLUT14L (1–217 nt) and GLUT3 (1–257 nt). Northern blot analysis was performed following the instructions from the manufacturer. The membranes were hybridized at 60°C. The probe labeling kit and hybridization reagents were purchased from Roche (Indianapolis, IN).