Isolation and characterization of eighteen polymorphic microsatellite loci for the topical seagrass, Syringodium isoetifolium

Eighteen polymorphic microsatellite loci were developed for the tropical seagrass, Syringodium isoetifolium. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Pacific Biosciences SMRT technology. The 18 loci were characterized in 24 individuals from the Indonesian archipelago. Observed levels of heterozygosity ranged from 0.14 to 0.91 with a mean of 3.7 alleles per locus. Four pairs of loci showed evidence of significant linkage disequilibrium and one locus deviated significantly from Hardy–Weinberg expectations.