Strain improvement studies on Kurthia species for the production of alkaline phosphatase

Alkaline phosphatase (E.C.3.1.3.1) or orthophosphoric monoester phosphohydrolase is a hydrolase enzyme responsible for dephosphorylation at alkaline pH. The enzymes are wide spread in environment. Applications of alkaline phosphatases in molecular biology, diagnostics, dairy, biosensors, agriculture and aquatic ecosystem have been studied (Suresh and Das, 2014; Bel-Ochi et al., 2013; Tekaya et al., 2013; Rankin et al., 2010; Durrieu et al., 2003; Jordan et al., 1995). The constant increase of alkaline phosphatase applications in diverse areas demands the enhanced production of alkaline phosphatase. Highest possible yield of the fermented product can be majorly obtained with utilized microorganism where the potential productivity of the microorganism can be attained by strain improvement. Strain improvement has been essential in the development of process for fermentation products. Improved strains can be achieved with mutations in natural strain and subsequent screening.