Cell biology of M-PEIs gene deliver prepared with radiation
2008
To study the feasibility of using polyethyleneimine nanoparticles(M-PEIs) prepared by radiation polym-erization as gene deliver,the cytotoxicity of M-PEIs was evaluated by MTS assay.The efficiency of short hairpin RNA(shRNA) transfection and its suppression of the targetted gene expression were investigated by RT-PCR and Western Blotting.It was found that the M-PEIs/shRNA complexes exhibited low cyto-toxicity in HCC.M-PEIs/shRNA also suppressed the target gene expression in liver cancer cell HepG2.The results indicate that M-PEIs have potential as a shRNA delivering agent and can be applied for anti-angiogenic tumor therapy.
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