Phytochemical Analysis and Anti-MicrobialActivities of Rosa Indica Collected fromKohat Pakistan

Objectives: The aim of the research work was to assess out the antimicrobial activity and phytochemicals in medicinal plant, Rosa indica. Methods: For phytochemical screening different reagents were used while antimicrobial i.e. antibacterial and antifungal activities were checked by employing various bacterial and fungal strains, using nutrient agar at 37◦C and agar tube dilution method, the stock solutions of crude and other three fractions were prepared in DMSO i.e. in 4mg/ 1ml ratio respectively. Results: All solvent soluble fractions of Rosa Indica showed superlative activity against Streptococcus pneumonia, Shigella flexneri, Proteus mirabilis, Pneumonia, Salmonella typhimurium, Vibia cholera, E. coli, Pneumonia aeruginosa but no activity was recorded against Proteus mirabilis by hexane and chloroform fraction. Similarly the crude extract exhibited complete anti-fungal activity against all fungal strains. Chloroform fraction was active in all fungal strains except Aspergillus fumigatus. The n-hexane fraction was active against Aspergillus fumigatus and Aspergillus Niger while n-butanol fractions exhibited best Antifungal activity against all fungal strains except Aspergillus Niger. The Rosa indica show best phytochemical results. Conclusion: The above mentioned result recommends that Rosa Indica have excellent anti-fungal as well as anti-bacterial properties and can be used for disease management and therapy. Beside this Rosa Indica have also gain important in scientific ethno-botanical studies because of their phyto-chemicals.