Evaluation of (GTG)5-PCR fingerprinting for characterization ofenterococci isolated from bryndza cheese
2005
A group of previously well-identified enterococci isolated from
a typical Slovakian sheep-milk product bryndza were
characterized by the (GTG)5-PCR fingerprinting in order to
evaluate discriminatory power of this method for identification
and strain-typing. Analysed group consisted of E. faecium (n =
76), E. faecalis (n = 25) and E. durans (n = 10) strains. They
were sampled from two bryndza-processing manufactories during
three monthly samplings performed successively (April, May and
June) in 2000 and 2001. Isolated strains were characterized and
identified by biotyping, ITS-PCR, ddlE.faecalis and
ddlE.faecium PCRs. Species identity of representative strains
selected from each (GTG)5-PCR fingerprint subcluster was
confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR
fingerprint profiles obtained from E. faecalis strains were
visually very close. Similarly, E. durans strains revealed
nearly identical fingerprints. E. faecium strains were
separated into two clearly separated clusters (30 %
similarity), but both these groups were confirmed as E. faecium
by all methods mentioned above. No clear correlation between
locality, sampling period and obtained fingerprint profiles was
revealed. Our results imply that (GTG)5-PCR fingerprinting is
useful for their identification at the species level, but not a
suitable tool for epidemiological studies nor for typing of
enterococcal strains. This work was supported by the Belgian
Federal Science Policy Office by a research fellowship for P.
S. obtained in the framework of the promotion of S&T
cooperation with Central and Eastern Europe.
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