Stimulation of Ovarian Maturation Using Serotonin (5-Hydroxytryptamine) Hormone on Banana Shrimp, Fenneropenaeus merguiensis (De Man, 1888)

2 Abstract: This study was conducted in order to investigate the potential of using serotonin, 5- hydroxytryptamine (5-HT) hormone to induce ovarian maturation on F. merguiensis. Control group consisted of two subgroups: a normal group and an eyestalk ablation group. 5-HT group consisted of two subgroups; one group treated with 20 µg hormones per gram body weight (5-HT20) and another group treated with 40 µg hormones per gram body weight (5-HT40). 100 µl of 5-HT solution was injected at first abdominal somite of shrimp at Day 1 and Day 11. After 20 days, the ovarian stage of shrimp was determined by physical observation using transmission light method and by histological analysis, conducted for further confirmation of ovarian stages. Oocytes diameter was measured under advance research microscope (Nikon Eclipse 80i) using NIS-Elements D 2.30 computer software. The mean size of oocytes was analyzed using One-Way ANOVA and Tukey Test at = 0.05. Results show that there is a significant difference between oocyte diameter of 5-HT treatment group and control group and no significant difference between both concentrations of 5-HT used. However, signal of maturation wasobserved from the 5-HT40 group (histological analysis) in 20 days experiment. This experiment suggests that 5-HT can induce faster maturation in F. merguiensis; however a further study mus be conducted to find the effect of 5-HT to the seed quantity and quality of F. merguiensis.