Significant enhancement of citric acid production by Yarrowia lipolytica immobilized in bacterial cellulose-based carrier

Abstract The aim of this study was to improve the yield of citric acid (CA), an industrially valuable metabolite, obtained during Yarrowia lipolytica yeast culturing. To this end, Y. lipolytica cells were immobilized on a novel bacterial cellulose (BC) based carrier and subjected to four subsequent cycles of fed-batch culturing. During the fermentation process, yeasts metabolic stability, glucose consumption and CA production were analyzed. The results of our study have shown that BC-immobilized yeasts utilized more glucose than free cells and that the metabolic activity of BC-immobilized cells and the resultant CA production remained on a stable level throughout 4 fermentation batches, while the drop in free cells’ metabolic stability and the consequent drop in CA production was observed with each subsequent batch. Also, the overall concentration of CA product was higher in immobilized vs. free yeasts (121-129 g/L vs. 99-110 g/L, respectively). The presented results indicate that the application of a BC carrier for Y. lipolytica culturing correlates not only with a higher yield of CA product but also with more stable and repeatable conditions of the biotechnological fermentation process. The results obtained in this study may find multiple biotechnological applications in which immobilization of various types of cells is required.