High-Performance Liquid Chromatographic Method for the Determination of Blasticidin S in Formulated Products with Photodiode Array Detection

An HPLC method with photodiode array detection is not only promising in distinguishing between the blasticidin S product and other antibiotic fungicide products that are falsely claimed to contain blasticidin S, but also for being capable of analyzing blasticidin S content in different formulations. The HPLC method detection limit was 0.05 μg/mL. The relative standard deviation (RSD) values of HPLC for the determination of blasticidin S in formulated products ranged from 0.70 to 2.83, and the RSD values for bioassay method ranged from 1.81 to 9.27%. This HPLC method may provide a useful monitoring technique for residue analysis. Keywords: HPLC; bioassay; antibiotic fungicide; blasticidin S; molecular weight; detection limit; sensitivity; selectivity