1H-NMR study of plantacyanin from spinach

Abstract High-resolution 1 H-NMR studies of spinach plantacyanin in its oxidized, reduced and apo-forms revealed that two histidines, one tryptophan and at least one methionine are very close to copper. These amino acids seem to be considered as possible ligands of copper in the protein. Spinach plantacyanin, as well as the cucumber protein, has the hydrophobic core around its copper.